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mouse anti mcherry  (Novus Biologicals)


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    Structured Review

    Novus Biologicals mouse anti mcherry
    Mouse Anti Mcherry, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mcherry/mCherry+Antibody+(235)/us12521412-882-46-48
    Average 94 stars, based on 27 article reviews
    mouse anti mcherry - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization.
    Article Snippet: Tools for acute manipulation of protein localization enable elucidation of spatiotemporally defined functions, but their reliance on exogenous triggers can interfere with cell physiology.. This limitation is particularly apparent for studying mitosis, whose highly choreographed events are sensitive to perturbations.. Here we exploit the serendipitous discovery of a phosphorylation-controlled, cell cycle-dependent localization change of the adaptor protein PLEKHA5 to develop a system for mitosis-specific protein recruitment to the plasma membrane that requires no exogenous stimulus.

    Article Title: Efficient cellular transformation via protein delivery through the protrusion-derived extracellular vesicles.
    Article Snippet: The following antibodies were used for western blotting (WB) and/or immunofluorescence staining (IF) at the following dilutions: mouse anti-CIP4 (BD, clone 21, 612556, 1:100 for IF, 1:1000 for WB), mouse anti-GAPDH (Santa Cruz, sc-166574, 1:2000 forWB), rabbit anti-WAVE2 (Cell signaling, clone D2C8, #3659, 1:100 for IF, 1:1000 for WB), rabbit anti-N-WASP (Cell signaling, clone 30D10, #4848, 1:1000 for WB), mouse anti-dynamin II (BD, #610263, 1:1000 for WB), mouse antiendophilin II (Santa Cruz, #sc-365704, 1:1000 for WB), rabbit anticlathrin light chain A (CLTA) (proteintech, #10852-1-AP, 1:1000 for WB), mouse anti-Rac1 (Millipore, 05-389, 1:4000 for WB), mouse antiRac1 (BD, #610651, 1:200 for WB of EV uptake, 1:400 for ELISA), rabbit anti-Cdc42 (Cell signaling, #2462, 1:1000 for WB), rabbit anti-HaloTag (Promega, #G9281, 1:1000 for WB, 1:1000 for IF, 1:1000 for ELISA), mouse anti-HaloTag (Promega, #G9211, 1:1000 for WB, 1:1000 for ELISA), mouse monoclonal anti-MIM (Abnova, #H00009788-M01, 1:1000 for WB), mouse anti-CD81 (Santa Cruz, #sc-166029, 1:2000 for WB), mouse anti-Annexin I (Santa Cruz, sc-12740, 1:1000 for WB), rabbit anti-EEA1 (Cell signaling, #3288, 1:100 for IF), mouse anti-Lamp1 (SantaCruz, #sc-20011,1:50 for IF),mouse anti-β-actin (SantaCruz, #sc47776, 1:3000 for WB), mouse anti-mCherry (Novus, #NBP1-96752, 1:1000 for IF), rabbit anti-GFP (MBL Life Science, #598, 1:4000 forWB), and rabbit anti-RFP (MBL Life Science, #PM005, 1:3000 for WB).

    Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
    Article Snippet: Mouse anti-PLEKHA5, Santa Cruz Biotechnology (sc-390311, RRID: N/A); rabbit anti-EGFR, Rockland (100-401-149, RRID: AB_2293276); mouse anti-actin, MP Bio (08691001, RRID: AB_2335127); mouse anti-V5, Bio-Rad (MCA1360GA, RRID: AB_567249); mouse anti-CD44, Cell Signaling Technology (3570, RRID: AB_2076465); rabbit anti-EEA1, Thermo Fisher (PA1-063A, RRID: AB_2096819); mouse anti-α tubulin, Sigma-Aldrich (T5168, RRID: AB_477579); rabbit anti-Histone H3, Cell Signaling Technology (4499, RRID: AB_10544537); mouse anti-GFP, Santa Cruz Biotechnology (sc-9996, RRID: AB_627695); mouse anti-mCherry, Novus Biologicals (NBP1-96752, RRID: AB_11034849); rabbit anti-phospho-Threonine/Tyrosine, Cell Signaling Technology (9381, RRID: AB_330301); goat anti-mouse HRP, Jackson ImmunoResearch Labs (115-035-146, RRID: AB_2307392); goat anti-rabbit HRP, Jackson ImmunoResearch Labs (111-035-144, RRID: AB_2307391); donkey anti-mouse Alexa Fluor 488, Invitrogen (A-21202, RRID: AB_141607).

    Article Title: Efficient cellular transformation via protein delivery through the protrusion-derived extracellular vesicles
    Article Snippet: HEK293 platA cells were transfected with pMXs-CIP4-mEos4b-IRES-HygR or pMXs-CIP4-mCherry-IRES-HygR using 293-fectin (Invitrogen) for 2 days to produce retrovirus, and the supernatant containing the retrovirus was filtered and infected with CIP4 KO PANC-1 cells in the presence of polybrene (Sigma, TR-1003) at 32 μg/ml.


    Incubation:

    Article Title: Nucleic acids and nucleic acid analogs for treating, preventing, and disrupting pathological polynucleotide-binding protein inclusions
    Article Snippet: Following water and TBS washes, membranes were blocked in Odyssey Blocking Buffer (Li-Cor) for 1 hr at room temperature. .. Membranes were then washed and incubated with primary antibodies in TBS-T (0.1% Tween) supplemented with 50% blocking buffer overnight at 4° C. Primary antibody dilutions used were as follows: rabbit anti-TDP43 (Proteintech, 1:1000), mouse anti-α-tubulin (Sigma-Aldrich, 1:10000), rabbit anti-Lamin B1 (Abcam, 1:5000), rabbit anti-GAPDH (Sigma-Aldrich, 1:10000), mouse anti-mCherry (Novus Biologicals, 1:1000). .. Following TBS-T washes, membranes were incubated with secondary antibodies (Li-Cor, IRDye 680/800, 1:10000) for 1 hr at room temperature.

    Article Title: A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies
    Article Snippet: After a semi-dry transfer of proteins from the SDS-PAGE gels to Immuno-Blot PVDF membrane (BIO-RAD) was carried out, the membranes were blocked in 5% nonfat powder milk (Research Products International) reconstituted in PBS for 1 h. After blocking, the membranes were incubated with the primary antibodies (mouse anti-eGFP, Living Colors A.v. .. Monoclonal Antibody JL-8; catalog # 632381; dilution 1:3000; mouse anti-mCherry (1C51), Novus; catalog #NBP1-96752; dilution 1:3000) overnight, washed with PBS plus 0.1% Tween (PBST), and incubated for 1 h with the secondary antibody (anti-mouse HRP-linked secondary antibody, Cell Signaling; catalog #7076; dilution 1:10,000). .. Chemiluminescence imaging was conducted using the ChemiDoc imaging system (BIO-RAD).

    Blocking Assay:

    Article Title: Nucleic acids and nucleic acid analogs for treating, preventing, and disrupting pathological polynucleotide-binding protein inclusions
    Article Snippet: Following water and TBS washes, membranes were blocked in Odyssey Blocking Buffer (Li-Cor) for 1 hr at room temperature. .. Membranes were then washed and incubated with primary antibodies in TBS-T (0.1% Tween) supplemented with 50% blocking buffer overnight at 4° C. Primary antibody dilutions used were as follows: rabbit anti-TDP43 (Proteintech, 1:1000), mouse anti-α-tubulin (Sigma-Aldrich, 1:10000), rabbit anti-Lamin B1 (Abcam, 1:5000), rabbit anti-GAPDH (Sigma-Aldrich, 1:10000), mouse anti-mCherry (Novus Biologicals, 1:1000). .. Following TBS-T washes, membranes were incubated with secondary antibodies (Li-Cor, IRDye 680/800, 1:10000) for 1 hr at room temperature.



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